To go to the North Carolina Diabetes Research Center Genomics and Proteomics Core, click here

Members in the Center for Precision Medicine bring together expertise in sample collection and archiving, genomics, transcriptomics, proteomics, metabolomics, microbial -omics, cell biology, and computational biology including integrated omics to understand the transition of normal physiology to dysregulated disease processes at the molecular level.

Scientific Projects

Below you find newly initiated collaborative projects that involve multiple investigators from the Center for Precision Medicine. For more information, please contact the investigators directly.

Systemic Metabolic Programming in a Nonhuman Primate Model of Metabolic Disease: Pilot study to characterize the impact of nutrition and diet on health.
Contacts: Laura Cox, Ph.D., Kim Reeves, Ph.D., Tony Reeves, Ph.D.

Biobanking: Development of a resource that includes biological samples linked to phenotypic data for research projects related to aging, cardiometabolic disease, bioenergetics, and immune function.
Contacts: Laura Cox, Ph.D., Kim Reeves, Ph.D., Tony Reeves, Ph.D.

The TOPS GENOME Registry: A new community resource for obesity research.
(link here to TOPS Genome Registry website)
Contacts: Michael Olivier, Ph.D., Hector Guillen, Ph.D.

Technologies

Advances and Applications of Next Generation DNA Sequencing Greg Hawkins, PhD Department of Biochemistry Co-Director, Cancer Genomics Shared Resource

High Throughput Sequencing
-Illumina Next Seq 500-
Genomics: whole genome sequencing, exome sequencing, and targeted sequencing
Transcriptomics: RNA-Seq, small RNA-Seq
Contacts: Laura Cox, Ph.D., Greg Hawkins, Ph.D.

Metagenomics: microbiome whole genome sequencing; Contact: Kim Reeves, Ph.D.
(Link to Omics 101 Video Presentation on new page)

Proteomics and Mass Spectrometry: How does it work, and what can it do? Michael Olivier, PhD Department of Internal Medicine – Molecular Medicine Director, Center for Precision Medicine

Mass Spectrometry – Thermo Orbitrap Lumos Tribrid LC-MS System
Proteomics: Tissues, blood products, exosomes
Contacts: Hector Guillen, Ph.D., Michael Olivier, Ph.D.
(Link to Video URL on new page)

Metabolomics: A Technology for Biomarker Discovery and Compound Measurement Andrew Bishop, PhD Department of Internal Medicine – Molecular Medicine

Mass Spectrometry – Thermo QExactive Orbitrap Gas Chromatograsphy (GC)-MS System
Metabolomics: Tissues, blood products, exosomes, CSF; microbiome
Contacts: Andrew Bishop, Ph.D., Biswapriya Misra, Ph.D
(Link to Omics 101 Video Presentation on new page)

Mass Spectrometry –
LECO Pegasus Gas
Chromatograsphy (GC)-MS System
Metabolomics, untargeted: Breath
Contact: Andrew Bishop, Ph.D.

Profiling Cell Heterogeneity within Complex Populations Yong Joo Ahn, MD Department of Internal Medicine – Molecular Medicine

Single Cell Analysis
Cell sorting
Western blot analysis
Contacts: Yong Joo Ahn, M.D., Reto Asmis, Ph.D.
RNA-Seq
Contact: Greg Hawkins, Ph.D.

Link to Omics 101 video presentation on new page

How is the genome regulated? Identifying novel DNA-binding proteins Hector Guillen, PhD Department of Internal Medicine – Molecular Medicine

HyCCAPP - Hybridization Capture of Chromatin-Associated Proteins for Proteomics
Analysis of Protein-DNA interactions
Contacts: Hector Guillen, Ph.D., Michael Olivier, Ph.D.
(Link to Omics 101 Video Presentation on new page)

Computational biology

High Throughput Sequence data analysis: DNA Seq, RNA Seq, small RNA Seq for human and model organisms including nonhuman primates, sheep, rats, and mice
Contacts: Jeannie Chan, Ph.D., Laura Cox, Ph.D.

Admixture Modeling: Identification of admixture and population history events (e.g. natural selection and drift) that influence modern population-level differences in allele frequencies.
Contact: Ellen Quillen, Ph.D.

Proteomics Data Analysis: Quantitative analysis of proteomic changes and posttranslational modifications in biological systems
Contacts: Michael Olivier, Ph.D., Ellen Quillen, Ph.D.

Differential gene expression and pathway analysis Jeannie Chan, PhD Department of Internal Medicine – Molecular Medicine

Network Analysis: Ingenuity Pathway Analysis and Weighted Gene Coexpression Network Analysis
Contacts: Jeannie Chan, Ph.D.,
Sobha Puppala, Ph.D.
(click here for the Video Presentation on new page)

Integrated Omics: Better Models for Better Biology Ellen Quillen, PhD Department of Internal Medicine – Molecular Medicine

Integrated Omics: Unbiased integration of untargeted genomics, transcriptomics, proteomics, and metabolomics data
Contacts: Laura Cox, Ph.D., Biswapriya Misra, Ph.D., Ellen Quillen, Ph.D., Michael Olivier, Ph.D.

(click here for video presentation on separate page)

Using Metagenomic Sequencing to Characterize our “Other Genome” Kim Reeves, PhD Department of Internal Medicine – Molecular Medicine

Integrated Microbiome omics: Unbiased integration of untargeted genomics, proteomics, and metabolomics data (click here for the Video Presentation on new page)
Contact: Kim Reeves, Ph.D.

Statistical Genetics: Identification of genetic variation associated with quantitative trait variation
Contacts: Sobha Puppala, Ph.D., Ellen Quillen, Ph.D.

Summer student intern Taylor Outlaw analyzing data

Modeling of Molecular Targets: Development and characterization of diagnostic antibodies, protein drug formulation and early phase small molecule drug discovery
Contact: Tony Reeves, Ph.D.